التوصيف المظهري والوراثي لإنزيمات البيتالاكتاميز لعزلات البكتريا المعوية المسببة لإنتان الدم في محافظة بابل، العراق

Phenotypic and Genotypic Characterization of β-lactamases Enterobacteriaceae Isolated from Bacteremia in Babylon Province, Iraq

البحث في الرسائل

الباحث: دعاء حسين عبدعلي مصيبح
المشرف: فاطمة معين عباس
الجامعة: جامعة بابل
الكلية / القسم: كلية العلوم للبنات / علوم الحياة
الشهادة: ماجستير
السنة: 2022
لغة الرسالة: انكليزي
الكلمات المفتاحية: Beta-lactamases ; Enterobacteriaceae ; Bacteremia ; Babylon Province

Abstract

The present study include 163 blood samples collected from patients with bacteremia in hospitals in the Babil province during the period from October 2020 to April 2021. Only 63 bacterial isolates (38.65 %) as positive results. Of these, 35 bacterial isolates (21.47%) are identified as Enterobacteriaceae and 28 bacterial isolates (17.17) belonged to following species (Streptococcus.spp, Staphylococcus.spp and Pseudomonas aeruginosa). And the study reveal that Klebsiella pneumoniae (22.85%), Proteus mirabilis (20%), Salmonella typhi (20%) and Escherichia.coli (17.14%) are the most common isolated bacteria as compared with other members of the Enterobacteriaceae. The study shows that the susceptibility of males (51.42%) and females (48.57%) to blood stream infection is nearly the same. The disk difussion test is used to determine the antibiotic susceptibility patterns of 35 bacterial isolates, which reveals that the majority of these isolates are highly resistant to the antibiotics tested, particularly beta-lactam antibiotics, with carbapenems (meropenem) having the lowest rate of resistance (17.14%). Phenotypic ESBL detection was carried out by disk approximation method, 9 isolates (25.71%) were beta lactamase producers. Polymerase Chain Reaction (PCR) results reveals that among the 35 bacterial isolates, 19 isolates had the TEM enzyme type, 21 isolates had the OXA-10 enzyme type, and 23 isolates had the CTX-M-1 enzyme type. Results of PCR show that TEM, OXA-10, and CTX-M-1 enzymes are highly prevalent among species of Enterobacteriaceae that are responsible for blood stream infections. In addition to that, the PCR test is considered an accurate genotypic method used for detection of the ESBL enzyme.type.

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