تأثير المرافق الإنزيمي Q10 في السمية السلوكية العصبية والنسجية المرضية المحدثة بكبريتات النحاس في نموذج أفراخ الدجاج

الباحث

شهد إسماعيل سعيد النعيمي

المشرف

يمامة زهير صالح العبدلي

الجامعة

جامعة الموصل

الكلية / القسم

كلية الطب البيطري / الأدوية والسموم البيطرية

الشهادة

ماجستير

السنة

2022

لغة الرسالة

عربي

الكلمات المفتاحية

المرافق الإنزيمي Q10 ; كبريتات النحاس ; السمية العصبية ; أفراخ الدجاج

A The aim of this study is to investigate the acute neurotoxic effects of copper sulphate and the effect of coenzyme Q-10 administration at different times on the copper sulphate toxicity on the neurological behavior and motor activity of chicks, as well as their relationship to the activity of cholinesterase and caspase-3, the level of oxidative stress, and the histopathological changes in the brain tissue of chicks .144 chicks were used in this study When copper sulphate was given orally to chicks, the LD50was 772 mg/kg. the response to different doses of copper sulphate was determined through neurobehavioral tests, which represented a delay in the start of movement and a significant decrease in the number of squares cut, and this decrease was directly proportional to the increase in the administered dose compared to the control. As for the posture correction test, the administered dose recorded a significant increase of 5%, 10%, and 15% in posture correction time compared to the control and the other groups As for the tensile immobility test, the groups treated with doses of 5%, 10%, and 15% of the median lethal dose recorded a significant and clear increase in the dormancy period of chicks compared to the control group.The neurobehavioral changes when giving both copper sulphate and Q-10 together in the chicks recorded a significant increase in the start time of movement, the number of squares cut by the chicks in the same group recorded a significant decrease compared to the control group, while in the tensile immobility test, the group treated with copper sulfate recorded a significant increase in dormancy period of chicks in this test. B When compared to the control group and copper sulphate group, Chicks treated with copper sulphate recorded a significant decrease in theperiod of onset of sleep with a decrease in the period of anaesthesia in this group, while the rest of the groups recorded a significant increase in period of onset of sleep in groups treated with coenzyme Q-10 alone or with copper sulphate compared to the control group. As for the length of sleep duration, the groups treated with the coenzyme Q-10 alone or with copper sulphate recorded a significant decrease in sleep duration of the chicks compared to the control group and copper sulphate group. In comparison to the control group, the coenzyme Q-10 group had a significantly lower onset of sleep and a shorter sleep duration. In comparison between the control group and the other groups, The level of total antioxidant capacity in liver tissue and brain tissue in groups treated with copper sulphate at all doses showed a significant decrease compared to the control group. In comparison to the control group, copper sulphate concentrations of 2.5 percent, 5%, 10%, and 15% of the median lethal dose resulted in a significant drop in glutathione and a significant increase in malondialdehyde At varying concentrations of the lethal dose, the level of inhibition of cholinesterase activity demonstrated a significant drop in cholinesterase activity compared to the control group, with an increase in caspase-3 activity in the liver and brain tissue compared to the control group. When coenzyme Q-10 was given with copper sulphate at different times, the pharmacological challenge revealed substantial changes in sleep onset and sleep duration length in chicks. Higher Caspase-3 activity in brain tissue was connected to increased oxidative stress in total, glutathione, and malondialdehyde in serum and brain tissue, while groups that received given coenzyme Q-10 with copper sulphate concurrently or before oxidative stress had less oxidative stress and histological alterations, less pathology in brain tissue The severity of degenerative alterations in chick brain tissue was related to the increase in copper sulphate concentration administered to chicks, according to the results of histological investigation. The cerebral cortex of the chicks treated with copper sulphate and later coenzyme Q10 revealed a rise and accumulation of glial cells, diffuse angioedema, edoema surrounding the axons, and vacuolization of glial cells, according to the results of the histological investigation. Glial cells, neurons, and blood vessels The cerebral cortex of the group given coenzyme Q10 and then copper sulphate had normal histological structure of neurons with angioedema surrounding the axons We concluded from this research that high concentrations of copper sulphate in the brain cause oxidative stress and histopathological alterations in the chicks in the open field, affecting their neurological behavior and motor activities. In addition, taking CoQ-10 at the same time as or before taking copper sulphate decreases copper sulphate toxicity. This has been demonstrated using oxidative stress markers, biochemical markers, histopathological alterations, and programmed death of brain cells.

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